Journal: Advanced Science

Article Title: Identification and Characterization of the Wilms Tumor Cancer Stem Cell

doi: 10.1002/advs.202206787

Figure Lengend Snippet: List of antibodies used in the experimental procedures (IF: immunofluorescence, WB: Western blot)

Article Snippet: CLEC4M polyclonal antibody , Proteintech Group # 22003 , 1:50 IF.

Techniques: Immunofluorescence, Western Blot, In Vivo

Journal: eLife

Article Title: A proteome-wide genetic investigation identifies several SARS-CoV-2-exploited host targets of clinical relevance

doi: 10.7554/eLife.69719

Figure Lengend Snippet:

Article Snippet: Transfected construct ( Homo sapiens ) , pCMV6-CD209 , Origene , Cat.# SC304915 , Plasmid for CD209 cDNA expression in cell-based binding assay.

Techniques: Transfection, Construct, Plasmid Preparation, Expressing, Cell Binding Assay, Recombinant, Staining, Flow Cytometry, Cell Culture, Software

Journal: International Immunology

Article Title: Identification and isolation of splenic tissue-resident macrophage sub-populations by flow cytometry

doi: 10.1093/intimm/dxy064

Figure Lengend Snippet: Characterization of macrophage sub-populations isolated by the enzyme cocktail. (A) Spleen cells were stained with monoclonal antibodies against CD64, MerTK, Tim4, Ly6C, CD11c, MHC class II, CD169 and SIGNR1; each sub-population of macrophages gated according to the strategy in was analyzed for their expression. Red histograms indicate staining by the antibodies, and filled histograms indicate staining by the isotype control. (B) Each sub-population of macrophage was sorted from the spleen of mice ( n = 5) and analyzed for relative gene expression to whole spleen cells by quantitative PCR.

Article Snippet: PE-conjugated anti-mouse SIGNR1 (REA125) and PE-conjugated isotype control (REA293) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).

Techniques: Isolation, Staining, Bioprocessing, Expressing, Control, Gene Expression, Real-time Polymerase Chain Reaction